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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which ends up in chromosome instability, and so could contribute to tumorigenesis. This protein was found to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential function in selling mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are explained. Two further splice variants were being reported but thorough nucleotide sequence information just isn't still obtainable.
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Blocking peptides are peptides that bind exclusively towards the concentrate on antibody and block antibody binding. These peptide usually consists of the epitope identified through the antibody.
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This more info guidebook outlines more doable optimization of mobile and immunoassay parameters to be sure the absolute best benefits are...
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This guidebook outlines more achievable optimization of cellular and immunoassay parameters to ensure the very best final results are...
Antibodies bound to the blocking peptide no longer bind into the epitope to the focus on protein. By comparing the staining within the blocked antibody as opposed to the antibody by itself, one can see which staining is precise.
Goal Information Cyclin E belongs to your really conserved cyclin family, whose associates are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation designs which lead to the temporal coordination of every mitotic occasion. Cyclin E varieties a complex with and functions being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and it is degraded as cells progress by S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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